PoplarV1, Main, Exploration, bibRecord, 001014

Cellulose Synthase Stoichiometry in Aspen Differs from Arabidopsis and Norway Spruce.

Identifieur interne : 001014 ( Main/Exploration ); précédent : 001013; suivant : 001015

Cellulose Synthase Stoichiometry in Aspen Differs from Arabidopsis and Norway Spruce.

Auteurs : Xueyang Zhang [Suède] ; Pia Guadalupe Dominguez [Suède] ; Manoj Kumar [Suède, Royaume-Uni] ; Joakim Bygdell [Suède] ; Sergey Miroshnichenko [Suède] ; Björn Sundberg [Suède] ; Gunnar Wingsle [Suède] ; Totte Niittyl [Suède]

Source :

Plant physiology [ 1532-2548 ] ; 2018.

RBID : pubmed:29760198

Descripteurs français

KwdFr :
- Arabidopsis (cytologie), Arabidopsis (enzymologie), Diffusion de rayonnements (MeSH), Glucosyltransferases (isolement et purification), Glucosyltransferases (métabolisme), Paroi cellulaire (enzymologie), Peptides (analyse), Peptides (métabolisme), Picea (cytologie), Picea (enzymologie), Populus (cytologie), Populus (enzymologie), Protéines d'Arabidopsis (métabolisme), Protéines végétales (isolement et purification), Protéines végétales (métabolisme), Protéomique (méthodes), Spécificité d'espèce (MeSH), Xylème (métabolisme).
MESH :
- analyse : Peptides.
- cytologie : Arabidopsis, Picea, Populus.
- enzymologie : Arabidopsis, Paroi cellulaire, Picea, Populus.
- isolement et purification : Glucosyltransferases, Protéines végétales.
- métabolisme : Glucosyltransferases, Peptides, Protéines d'Arabidopsis, Protéines végétales, Xylème.
- méthodes : Protéomique.
- Diffusion de rayonnements, Spécificité d'espèce.

English descriptors

KwdEn :
- Arabidopsis (cytology), Arabidopsis (enzymology), Arabidopsis Proteins (metabolism), Cell Wall (enzymology), Glucosyltransferases (isolation & purification), Glucosyltransferases (metabolism), Peptides (analysis), Peptides (metabolism), Picea (cytology), Picea (enzymology), Plant Proteins (isolation & purification), Plant Proteins (metabolism), Populus (cytology), Populus (enzymology), Proteomics (methods), Scattering, Radiation (MeSH), Species Specificity (MeSH), Xylem (metabolism).
MESH :
- chemical , analysis : Peptides.
- chemical , isolation & purification : Glucosyltransferases, Plant Proteins.
- chemical , metabolism : Arabidopsis Proteins, Glucosyltransferases, Peptides, Plant Proteins.
- cytology : Arabidopsis, Picea, Populus.
- enzymology : Arabidopsis, Cell Wall, Picea, Populus.
- metabolism : Xylem.
- methods : Proteomics.
- Scattering, Radiation, Species Specificity.

Abstract

Cellulose is synthesized at the plasma membrane by cellulose synthase complexes (CSCs) containing cellulose synthases (CESAs). Genetic analysis and CESA isoform quantification indicate that cellulose in the secondary cell walls of Arabidopsis (Arabidopsis thaliana) is synthesized by isoforms CESA4, CESA7, and CESA8 in equimolar amounts. Here, we used quantitative proteomics to investigate whether the CSC model based on Arabidopsis secondary cell wall CESA stoichiometry can be applied to the angiosperm tree aspen (Populus tremula) and the gymnosperm tree Norway spruce (Picea abies). In the developing xylem of aspen, the secondary cell wall CESA stoichiometry was 3:2:1 for PtCESA8a/b:PtCESA4:PtCESA7a/b, while in Norway spruce, the stoichiometry was 1:1:1, as observed previously in Arabidopsis. Furthermore, in aspen tension wood, the secondary cell wall CESA stoichiometry changed to 8:3:1 for PtCESA8a/b:PtCESA4:PtCESA7a/b. PtCESA8b represented 73% of the total secondary cell wall CESA pool, and quantitative polymerase chain reaction analysis of CESA transcripts in cryosectioned tension wood revealed increased PtCESA8b expression during the formation of the cellulose-enriched gelatinous layer, while the transcripts of PtCESA4, PtCESA7a/b, and PtCESA8a decreased. A wide-angle x-ray scattering analysis showed that the shift in CESA stoichiometry in tension wood coincided with an increase in crystalline cellulose microfibril diameter, suggesting that the CSC CESA composition influences microfibril properties. The aspen CESA stoichiometry results raise the possibility of alternative CSC models and suggest that homomeric PtCESA8b complexes are responsible for cellulose biosynthesis in the gelatinous layer in tension wood.

DOI: 10.1104/pp.18.00394
PubMed: 29760198
PubMed Central: PMC6053019

Affiliations:

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<term>Arabidopsis Proteins (metabolism)</term>
<term>Cell Wall (enzymology)</term>
<term>Glucosyltransferases (isolation & purification)</term>
<term>Glucosyltransferases (metabolism)</term>
<term>Peptides (analysis)</term>
<term>Peptides (metabolism)</term>
<term>Picea (cytology)</term>
<term>Picea (enzymology)</term>
<term>Plant Proteins (isolation & purification)</term>
<term>Plant Proteins (metabolism)</term>
<term>Populus (cytology)</term>
<term>Populus (enzymology)</term>
<term>Proteomics (methods)</term>
<term>Scattering, Radiation (MeSH)</term>
<term>Species Specificity (MeSH)</term>
<term>Xylem (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Arabidopsis (cytologie)</term>
<term>Arabidopsis (enzymologie)</term>
<term>Diffusion de rayonnements (MeSH)</term>
<term>Glucosyltransferases (isolement et purification)</term>
<term>Glucosyltransferases (métabolisme)</term>
<term>Paroi cellulaire (enzymologie)</term>
<term>Peptides (analyse)</term>
<term>Peptides (métabolisme)</term>
<term>Picea (cytologie)</term>
<term>Picea (enzymologie)</term>
<term>Populus (cytologie)</term>
<term>Populus (enzymologie)</term>
<term>Protéines d'Arabidopsis (métabolisme)</term>
<term>Protéines végétales (isolement et purification)</term>
<term>Protéines végétales (métabolisme)</term>
<term>Protéomique (méthodes)</term>
<term>Spécificité d'espèce (MeSH)</term>
<term>Xylème (métabolisme)</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Peptides</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Glucosyltransferases</term>
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<term>Peptides</term>
<term>Plant Proteins</term>
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<term>Picea</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Arabidopsis</term>
<term>Picea</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Arabidopsis</term>
<term>Paroi cellulaire</term>
<term>Picea</term>
<term>Populus</term>
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<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Arabidopsis</term>
<term>Cell Wall</term>
<term>Picea</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Glucosyltransferases</term>
<term>Protéines végétales</term>
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<term>Peptides</term>
<term>Protéines d'Arabidopsis</term>
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<front><div type="abstract" xml:lang="en">Cellulose is synthesized at the plasma membrane by cellulose synthase complexes (CSCs) containing cellulose synthases (CESAs). Genetic analysis and CESA isoform quantification indicate that cellulose in the secondary cell walls of Arabidopsis (<i>Arabidopsis thaliana</i>
) is synthesized by isoforms CESA4, CESA7, and CESA8 in equimolar amounts. Here, we used quantitative proteomics to investigate whether the CSC model based on Arabidopsis secondary cell wall CESA stoichiometry can be applied to the angiosperm tree aspen (<i>Populus tremula</i>
) and the gymnosperm tree Norway spruce (<i>Picea abies</i>
). In the developing xylem of aspen, the secondary cell wall CESA stoichiometry was 3:2:1 for PtCESA8a/b:PtCESA4:PtCESA7a/b, while in Norway spruce, the stoichiometry was 1:1:1, as observed previously in Arabidopsis. Furthermore, in aspen tension wood, the secondary cell wall CESA stoichiometry changed to 8:3:1 for PtCESA8a/b:PtCESA4:PtCESA7a/b. PtCESA8b represented 73% of the total secondary cell wall CESA pool, and quantitative polymerase chain reaction analysis of CESA transcripts in cryosectioned tension wood revealed increased <i>PtCESA8b</i>
 expression during the formation of the cellulose-enriched gelatinous layer, while the transcripts of <i>PtCESA4</i>
, <i>PtCESA7a/b</i>
, and <i>PtCESA8a</i>
 decreased. A wide-angle x-ray scattering analysis showed that the shift in CESA stoichiometry in tension wood coincided with an increase in crystalline cellulose microfibril diameter, suggesting that the CSC CESA composition influences microfibril properties. The aspen CESA stoichiometry results raise the possibility of alternative CSC models and suggest that homomeric PtCESA8b complexes are responsible for cellulose biosynthesis in the gelatinous layer in tension wood.</div>
</front>
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<Month>03</Month>
<Day>13</Day>
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<Day>01</Day>
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<Title>Plant physiology</Title>
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<ArticleTitle>Cellulose Synthase Stoichiometry in Aspen Differs from Arabidopsis and Norway Spruce.</ArticleTitle>
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</Pagination>
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<Abstract><AbstractText>Cellulose is synthesized at the plasma membrane by cellulose synthase complexes (CSCs) containing cellulose synthases (CESAs). Genetic analysis and CESA isoform quantification indicate that cellulose in the secondary cell walls of Arabidopsis (<i>Arabidopsis thaliana</i>
) is synthesized by isoforms CESA4, CESA7, and CESA8 in equimolar amounts. Here, we used quantitative proteomics to investigate whether the CSC model based on Arabidopsis secondary cell wall CESA stoichiometry can be applied to the angiosperm tree aspen (<i>Populus tremula</i>
) and the gymnosperm tree Norway spruce (<i>Picea abies</i>
). In the developing xylem of aspen, the secondary cell wall CESA stoichiometry was 3:2:1 for PtCESA8a/b:PtCESA4:PtCESA7a/b, while in Norway spruce, the stoichiometry was 1:1:1, as observed previously in Arabidopsis. Furthermore, in aspen tension wood, the secondary cell wall CESA stoichiometry changed to 8:3:1 for PtCESA8a/b:PtCESA4:PtCESA7a/b. PtCESA8b represented 73% of the total secondary cell wall CESA pool, and quantitative polymerase chain reaction analysis of CESA transcripts in cryosectioned tension wood revealed increased <i>PtCESA8b</i>
 expression during the formation of the cellulose-enriched gelatinous layer, while the transcripts of <i>PtCESA4</i>
, <i>PtCESA7a/b</i>
, and <i>PtCESA8a</i>
 decreased. A wide-angle x-ray scattering analysis showed that the shift in CESA stoichiometry in tension wood coincided with an increase in crystalline cellulose microfibril diameter, suggesting that the CSC CESA composition influences microfibril properties. The aspen CESA stoichiometry results raise the possibility of alternative CSC models and suggest that homomeric PtCESA8b complexes are responsible for cellulose biosynthesis in the gelatinous layer in tension wood.</AbstractText>
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Cellulose Synthase Stoichiometry in Aspen Differs from Arabidopsis and Norway Spruce.

Cellulose Synthase Stoichiometry in Aspen Differs from Arabidopsis and Norway Spruce.

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